AMEI's Current Trends in Diagnosis & Treatment

Register      Login

VOLUME 1 , ISSUE 1 ( January-June, 2017 ) > List of Articles


Evaluating the Renoprotective Activity of 4-Methylthiobutyl Isothiocyanate against 7,12-Dimethylbenz(α) anthracene generated Radical Stress in Male Wistar Rats

Rohit Arora, Rahul Mannan, Sakshi Bhushan, Saroj Arora

Citation Information : Arora R, Mannan R, Bhushan S, Arora S. Evaluating the Renoprotective Activity of 4-Methylthiobutyl Isothiocyanate against 7,12-Dimethylbenz(α) anthracene generated Radical Stress in Male Wistar Rats. Curr Trends Diagn Treat 2017; 1 (1):10-14.

DOI: 10.5005/jp-journals-10055-0003

License: CC BY 3.0

Published Online: 01-06-2017

Copyright Statement:  Copyright © 2017; The Author(s).



An increasing environmental pollution has elevated the number of deleterious pollutants present in the atmosphere. Among these, polyaromatic hydrocarbons, such as 7,12-dimethylbenz(α)anthracene (DMBA) have posed serious threat to the health of both animals and plants. Most of these compounds are genotoxic in nature and an elevated exposure of these carcinogens increases the risk of initiation and promotion of cancer. The low efficacy, specific target sites, and large number of side effects of the available drugs have encouraged researchers to look for an alternate drug with high biological potency. The best alternate for this is the use of medicinal plants and especially their phytochemicals, such as glucosinolate hydrolytic products. A large number of glucosinolate hydrolytic products are available in different plant species, but sulforaphane has emerged as the most potent among them. This important compound is interconverted to 4-methylthiobutyl isothiocyanate (4-MTBITC) or erucin in the body of an organism. In contrast to sulforaphane, not much work has been done on the biological properties of 4-MTBITC. The current study, therefore, focuses on evaluating the renoprotective activity of 4-MTBITC on DMBA induced nephrotoxicity in male Wistar rats.

Materials and methods

The bioprotective efficacy of erucin was evaluated in male Wistar rats treated with DMBA using the levels of serum creatinine, urea, sodium, and potassium.


The treatment with erucin significantly ameliorated the DMBA induced stress in male Wistar rats.


Erucin or 4-MTBITC has the potential to impart protection to male Wistar rats against genotoxic carcinogen (DMBA) induced stress.

How to cite this article

Arora R, Kukreja S, Mannan R, Bhushan S, Arora S. Evaluating the Renoprotective Activity of 4-Methylthiobutyl Isothiocyanate against 7,12-Dimethylbenz(α)anthracene generated Radical Stress in Male Wistar Rats. Curr Trends Diagn Treat 2017;1(1):10-14.

  1. Oncogenic signaling pathways activated in DMBA-induced mouse mammary tumors. Toxicol Pathol 2005 Oct 1;33(6):726-737.
  2. Sources, distribution, and toxicity of polycyclic aromatic hydrocarbons. J Environ Health 2011 May 1;73(9):22.
  3. Additive roles of XPA and MSH2 genes in UVB-induced skin tumorigenesis in mice. DNA Repair 2002 Nov 3;1(11s):935-940.
  4. The role of dietary extra virgin olive oil and corn oil on the alteration of epigenetic patterns in the rat DMBA-induced breast cancer model. PLoS One 2015 Sep 24;10(9):e0138980.
  5. Interactions of betulinic acid with xenobiotic metabolizing and antioxidative enzymes in DMBA-treated Sprague Dawley female rats. Free Radic Biol Med 2013 Dec 31;65:131-142.
  6. Antioxidant role of Holothuria atra extract against nephrotoxicity induced by 7,12-dimethylbenz (α) anthracene in male albino rats. Int J 2015;3(2):275-287.
  7. 7,12-Dimethylbenz (α) anthracene-induced genotoxicity on bone marrow cells from mice phenotypically selected for low acute inflammatory response. DNA Repair 2016 Jan 31;37:43-52.
  8. Current applications and future potential for bioinorganic chemistry in the development of anticancer drugs. Drug Discov Today 2009 Dec 31;14(23):1089-1097.
  9. Cardiotoxicity of anticancer drugs: the need for cardio-oncology and cardio-oncological prevention. J Natl Cancer Inst 2010 Jan 6;102(1):14-25.
  10. Profiling glucosinolates, flavonoids, alkaloids, and other secondary metabolites in tissues of Azima tetracantha L. (Salvadoraceae). J Agric Food Chem 2004 Sep 22;52(19):5856-5862.
  11. Bio-protective effects of glucosinolates – a review. LWT Food Sci Technol 2009 Dec 31;42(10):1561-1572.
  12. Preclinical and clinical evaluation of sulforaphane for chemoprevention in the breast. Carcinogenesis 2007 Jul 1;28(7):1485-1490.
  13. Inhibition of bladder cancer by broccoli isothiocyanates sulforaphane and erucin: characterization, metabolism, and interconversion. Mol Nutr Food Res 2012 Nov 1;56(11):1675-1687.
  14. Conventional and modified hydrodistillation method for the extraction of glucosinolate hydrolytic products: a comparative account. SpringerPlus 2016 Dec 1;5(1):1-4.
  15. Hepatic dysfunction induced by 7,12-dimethylbenz (α) anthracene and its obviation with erucin using enzymatic and histological changes as indicators. PLoS One 2014 Nov 12;9(11):e112614.
  16. To analyze the amelioration of phenobarbital induced oxidative stress by erucin, as indicated by biochemical and histological alterations. Anticancer Agents Med Chem 2016 Apr 15.
  17. Drug metabolism in liver disease: activity of hepatic microsomal metabolizing enzymes. Clin Pharmacol Ther 1979 Oct;26(4):483-492.
  18. Renal function in the elderly (>70 years old) measured by means of iohexol clearance, serum creatinine, serum urea and estimated clearance. Scand J Urol Nephrol 2004 Jan 1;38(1):73-77.
  19. Using serum creatinine to estimate glomerular filtration rate: accuracy in good health and in chronic kidney disease. Ann Intern Med 2004 Dec 21;141(12):929-937.
  20. A more accurate method to estimate glomerular filtration rate from serum creatinine: a new prediction equation. Ann Intern Med 1999 Mar 16;130(6):461-470.
  21. Protective effects of Allium paradoxum against gentamicin-induced nephrotoxicity in mice. Food Funct 2012;3(1):28-29.
  22. Electrogenic sodium pump in nerve and muscle cells. Physiol Rev 1972 Jul 1;52(3):563-594.
  23. Links between dietary salt intake, renal salt handling, blood pressure, and cardiovascular diseases. Physiol Rev 2005 Apr 1;85(2):679-715.
  24. Saffron suppresses oxidative stress in DMBA-induced skin carcinoma: a histopathological study. Acta Histochem 2010 Jul 31;112(4):317-327.
  25. Cancer related mutations in NRF2 impair its recognition by Keap1-Cul3 E3 ligase and promote malignancy. Proc Natl Acad Sci USA 2008 Sep 9;105(36):13568-13573.
  26. Role of nrf2 in oxidative stress and toxicity. Annu Rev Pharmacol Toxicol 2013;53:401-426.
  27. Comparative action of 1,4-phenylenebis (methylene) selenocyanate and its metabolites against 7,12-dimethylbenz [α] anthracene-DNA adduct formation in the rat and cell proliferation in rat mammary tumor cells. Chem Biol Interact 2003 Oct 25;146(2):179-190.
  28. Interactive effects of nrf2 genotype and oltipraz on benzo[a]pyrene–DNA adducts and tumor yield in mice. Carcinogenesis 2003 Mar 1;24(3):461-467.
  29. Inhibition of 7,12-dimethylbenz (α) anthracene-induced skin tumorigenesis in C57BL/6 mice by sulforaphane is mediated by nuclear factor E2–related factor 2. Cancer Res 2006 Aug 15;66(16):8293-8296.
  30. Sulforaphane protects kidneys against ischemia-reperfusion injury through induction of the Nrf2-dependent phase 2 enzyme. Biochem Pharmacol 2008 Jun 1;75(11):2214-2223.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.