AMEI's Current Trends in Diagnosis & Treatment

Register      Login

VOLUME 1 , ISSUE 1 ( January-June, 2017 ) > List of Articles

ORIGINAL RESEARCH

Study of Serum Superoxide Dismutase and Malondialdehyde Levels during Normal Pregnancy

Manjeet Kaur, Roopam Bassi, Kiran Mehta, Deepinder Kaur

Citation Information : Kaur M, Bassi R, Mehta K, Kaur D. Study of Serum Superoxide Dismutase and Malondialdehyde Levels during Normal Pregnancy. Curr Trends Diagn Treat 2017; 1 (1):1-5.

DOI: 10.5005/jp-journals-10055-0001

License: CC BY 3.0

Published Online: 00-06-2017

Copyright Statement:  Copyright © 2017; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Introduction

Pregnancy is accompanied by a high-energy demand of all body functions and an increased oxygen requirement, which can give rise to increased levels of oxidative stress.

Aims and objectives

The present study was done to assess the levels of oxidative stress in healthy pregnant females by estimating serum superoxide dismutase (SOD) and malondialdehyde (MDA) levels and comparing these with nonpregnant females.

Materials and methods

The study was conducted on 88 female subjects in the age group of 20 to 40 years, who were placed in two groups. Group I consisted of 50 healthy nonpregnant females. Group II consisted of healthy pregnant females further subdivided into three subgroups: Group IIa (1st trimester) n = 38, group IIb (2nd trimester) n = 32, and group IIc (3rd trimester) n = 30. The same subjects were followed up till the 3rd trimester. There was a drop in the number of subjects from 38 to 32 in the 2nd trimester and from 32 to 30 in the 3rd trimester due to noncompliance and development of complications of pregnancy. The subjects were assessed for hemoglobin (Hb), MDA, a marker for lipid peroxidation, and SOD, an antioxidant enzyme along with certain physiological parameters like height, weight, body mass index, and blood pressure in the three trimesters.

Results

The levels of SOD and MDA were significantly raised with the progression of pregnancy. There was a fall in Hb levels in the 3rd trimester. But no significant correlation could be drawn between the measured parameters in all the trimesters.

Conclusion

Pregnancy is definitely associated with oxidative stress to the body. Both peroxidation and antioxidation reactions are enhanced during pregnancy. With these findings, we therefore conclude that antioxidant supplements should be prescribed in early pregnancy to prevent the overwhelming of oxidative stress in pregnant females.

How to cite this article

Bassi R, Sharma S, Mehta K, Kaur M, Kaur D. Study of Serum Superoxide Dismutase and Malondialdehyde Levels during Normal Pregnancy. Curr Trends Diagn Treat 2017;1(1):1-5.


  1. Dietary antioxidant flavonoids and coronary heart disease. J Assoc Physicians India 1996 Oct;44(10):698-708.
  2. Role of reactive oxygen species in gynaecological diseases. Review article. Reprod Med Biol 2004 Dec;3(4):177-199.
  3. Free radicals in the physiological control of cell function. Physiol Rev 2002 Jan;82(1):47-95.
  4. Active labor is associated with increased oxidisibility of serum lipids ex vivo. BJOG 2002;109:938-941.
  5. Oxidative stress in the placenta. Histochem Cell Biol 2004 Oct;122(4):369-382.
  6. Evaluation of pro-oxidants and antioxidants in pre-eclampsia. J Obstet Gynaecol India 2003;53(5):445-448.
  7. Analysis of the level of free radical lipid peroxidation and antioxidative system activity during different pregnancy weight gain and multifetal pregnancy. Acta Med Lituanica 2005;12(2):8-13.
  8. Lipid peroxidation and antioxidant enzymes in gestational diabetes. Indian J Physiol Pharmacol 2003 Oct;47(4):441-446.
  9. Changes in maternal lipid peroxidation levels and antioxidant enzymatic activities before and after delivery. J Nippon Med Sch 2000 Dec;67(6):434-443.
  10. Hypoxia and oxygen toxicity. Adv Neurol 1979;26:255-259.
  11. Increase in endothelial nitric oxide synthase and endothelin – 1 mRNA expression in human placenta during gestation. Eur J Obstet Gynecol Reprod Biol 2001 Aug;97(2):163-167.
  12. Total antioxidant activity in normal pregnancy. Online J Health Allied Sci 2009;8(2):8.
  13. Serum lipid peroxide in cerebrovascular disorders determined by a new colourimetric method. Clin Chem Acta 1998 Nov;90(1):37-43.
  14. Involvement of superoxide anion radical in the autooxidation of pyrogallol and a convenient assay of superoxide dismutases. Eur J Biochem 1974 Sep;47(3):469-474.
  15. Assay of superoxide dismutases activity in animal tissue. J Biosci 1988 Sep;13(3):305-315.
  16. Oxidative stress II. Oxidants and antioxidants. London: Academic Press; 1991.
  17. The physiology of human placenta. London: UCL Press Limited; 1993. p. 164.
  18. Causes of oxidative stress in the pre and perinatal period. Biol Neonate-Fetal Neonatal Res 2002;81(3):146-157.
  19. Oxidants, antioxidants and carcinogenesis. Indian J Exp Biol 2002 Nov;40(11):1220-1221.
  20. Differential localization of superoxide dismutase isoforms in placental villous tissue of normotensive, pre-eclamptic and intrauterine growth-restricted pregnancies. J Histochem Cytochem 1997 Oct;45(10):1433-1438.
  21. Total free radical trapping antioxidant potential in normal pregnancy. J Obstet Gynecol Ind 2004 May-Jun;54(3):249-250.
  22. J. Maternal physiology during pregnancy. In: DeCherney, A.H.; Pernoll, M.L., editors. Current obstetrics and gynaecology diagnosis and treatment. 8th ed. Upper Saddle River, NJ: Prentice Hall International Inc.; 1994. p. 148-149.
  23. Selenium and malondialdehyde content and glutathione peroxidase activity in maternal and cord blood and amniotic fluid. Biol Trace Elem Res 2000 Jan;73(1):47-54.
  24. Levels of lipid peroxides in uncomplicated pregnancy: a review of the literature. Reprod Toxicol 1999 Sep-Oct;13(5):347-352.
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.